RNA, Guide, CRISPR-Cas Systems

**Definition:** A component of CRISPR-Cas SYSTEMS. Cas endodeoxyribonucleases assemble with a guide RNA complex, a hybrid of CRISPR RNA (crRNA) and transactivating crRNA (tracrRNA) molecules, to form an active complex that cleaves DNA. crRNA and tracrRNA can be synthetically fused into a single RNA molecule, namely single guide RNA. Synthetic sgRNA is used with CRISPR-Cas SYSTEMS for targeted GENE EDITING.

**Tree numbers:** - D13.444.735.790.552.563

**Synonyms:** - RNA, Guide (CRISPR-Cas Systems) - Guide RNA, CRISPR-Cas Systems - Guide RNA (CRISPR-Cas Systems)