Freeze Substitution

**Definition:** A modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low temperature.

**Tree numbers:** - E01.370.225.500.620.760.160.260.270 - E01.370.225.750.600.760.160.260.270 - E02.792.156.260.270 - E05.200.500.620.760.160.260.270 - E05.200.750.600.760.160.260.270 - E05.760.156.260.270