Two-Hybrid System Techniques

**Definition:** Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.

**Tree numbers:** - E05.393.220.870 - E05.601.690.650 - E05.601.870

**Synonyms:** - Two-Hybrid Assay - Two-Hybrid Method - Two-Hybrid System Technics