Chromatin Immunoprecipitation

**Definition:** A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

**Tree numbers:** - E05.393.170 - E05.478.605.160

**Synonyms:** - Immunoprecipitation, Chromatin