Enzyme-linked Immunosorbent Spot Assay

**Semantic type:** Laboratory Procedure

**Definition:** A highly sensitive immunoassay capable of detecting secreted materials from individual cells upon activation. If the material of interest is secreted in response to a particular stimulus, then the material will bind to a specific immobilized antibody. Binding is then verified and visualized by applying other antibodies that have been tagged in some way.

**Synonyms:** - ELISPOT - ELISPOT - ELISpot