NOMe-Seq

**Semantic type:** Molecular Biology Research Technique

**Definition:** A DNA sequencing technique that can both determine DNA methylation status and distinguish non-nucleosomal DNA from nucleosome-associated DNA. This technique subjects samples to both bisulfite treatment to modify unmethylated cytosines and GpC methyltransferase treatment to identify non-nucleosomal DNA regions. The treated samples are then analyzed using next-generation sequencing techniques.

**Synonyms:** - NOME-Seq - Nucleosome Occupancy and Methylome Sequencing