Bisulfite Sequencing

**Semantic type:** Molecular Biology Research Technique

**Definition:** A DNA sequencing technique that can differentiate cytosine from 5-methylcytosine in a DNA sample. First, a denatured DNA sample is treated with bisulfite which converts non-methylated cytosine to uracil. Next, the sample is amplified using a PCR method that does not discriminate between non-methylated and methylated sequences. The amplified DNA is subjected to nucleotide sequencing. The resulting sequence is compared to an identical control sample of DNA that was not treated with bisulfite. Unmethylated cytosines will be displayed as cytosines in the control sample and as thymines in the bisulfite-treated sample.

**Synonyms:** - BS-Seq - Bisulfite-Seq - Bisulfite-Seq - Bisulfite-Seq (sequencing following treatment of DNA with bisulfite to convert cytosine residues to uracil) - Bisulphite Conversion Followed by Sequencing - DNA Methylation Sequencing - Methyl-C-Seq - MethylC-Seq - Methylation Sequencing - Methylation Sequencing