Immobilized Metal Affinity Chromatography with Phosphopeptide Enrichment

**Semantic type:** Laboratory Procedure

**Definition:** A method to purify phosphoproteins or phosphopeptides from a mixture that leverages their affinity to ferric ion-charged NTA-agarose beads. The mixture is loaded on the column at low pH and the phosphorylated proteins or peptides are eluted using alkaline buffers containing edetate at a pH range of 10-11 or phosphate-containing buffers.

**Synonyms:** - Phosphopeptide Enrichment