Northern Blotting

**Semantic type:** Laboratory Procedure

**Definition:** A common molecular biology procedure for detection of RNA molecules. RNA extracted from a sample was denatured and separated by slab gel electrophoresis, then blotted on to a nitrocellulose or nylon sheet and hybridized with labeled DNA or RNA that is complementary to the desired RNA, whose presence is subsequently indicated by autoradiography or other visualization techniques.

**Synonyms:** - Blotting, Northern - Northern Blot