Southern Blotting

**Semantic type:** Laboratory Procedure

**Definition:** A technique for the detection of specific DNA fragments that have been separated by polyacrylamide-gel electrophoresis, transferred to a nitrocellulose or other type of paper or nylon membrane, and detected by hybridization and visualization with a labeled probe.

**Synonyms:** - Blotting, Southern - Southern - Southern Blot - Southern Blot Hybridization - Southern blot