Pulsed-Field Gel Electrophoresis

**Semantic type:** Molecular Biology Research Technique

**Definition:** Pulsed-field gel electrophoresis (PFGE) is a method used to separate proteins or high molecular weight linear DNA fragments that are greater than 50 kilobases (kb) in size. Fragments are separated by pulsing an electrical field across an agarose gel rather than applying the constant field used in conventional electrophoresis.

**Synonyms:** - Electrophoresis, Gel, Pulsed-Field - Fractionation, Pulse Field - PFGE - PULSED-FIELD GEL ELECTROPHORESIS