Intracellular Cytokine Stain Flow Cytometric Assay

**Semantic type:** Laboratory Procedure

**Definition:** A technique that is used with flow cytometry to measure cytokine production in isolated cells. Cells are stimulated with an antigen or mitogen, and cytokine secretion is blocked using protein transport inhibitors. The cells are then stained with fluorochrome labeled monoclonal antibodies that target surface markers, fixed and permeabilized, and stained with fluorochrome labeled anti-cytokine monoclonal antibodies prior to analysis by flow cytometry.

**Synonyms:** - ICS - ICS - INTRACELLULAR CYTOKINE STAIN FLOW CYTOMETRIC ASSAY - Intracellular Cytokine Staining