Bicinchoninic Acid Assay

**Semantic type:** Laboratory Procedure

**Definition:** A copper-based colorimetric assay for total protein quantification. This assay relies on the formation of a Cu2+-protein complex in a basic environment, followed by reduction of the Cu2+ to Cu+, causing a change of color from green to purple, with a strong absorbance at 562nm.

**Synonyms:** - BCA - BCA - Smith Assay