cDNA Library Construction

**Semantic type:** Molecular Biology Research Technique

**Definition:** Methods for building collections of cloned DNA consisting of DNA copies of all the mRNAs produced by a cell type. cDNAs are generated using reverse transcriptase and either an oligo-dT or random primer. The copies are inserted into a bacterial plasmid or bacteriophage vector. The library can be maintained as a pooled population or as an arrayed library.

**Synonyms:** - library selection - library_selection