Quantitative Oblique Back-Illumination Microscopy

**Semantic type:** Laboratory Procedure

**Definition:** A visible light microscopy method that provides nanometer resolution in thick specimens. Light is supplied from the same side of the sample as the detection optics and internal light scattering produces a virtual light source from the opposite side of the imaging plane. This epi-illumination is used to produce differential phase contrast to remove out-of-focus information and permit tomographic sectioning.

**Synonyms:** - qOBM