Patient-Derived Micro-organospheres

**Semantic type:** Intellectual Product

**Definition:** An experimental tumor model comprised of a suspension culture of patient-derived cells in spherical droplets embedded in an artificial extracellular matrix (Matrigel). To generate the micro-organospheres, tumor samples or normal tissue fragments derived from cancer patients are minced and subjected to dissociation by gentle agitation in a enzyme solution, the sample is filtered and cell numbers and viability are determined. The cells are then suspended in liquid Matrigel at the desired concentration, separated into droplets using an oil-based microfluidics chip system, excess oil is removed, the Matrigel solidifies and the cell-containing droplets are placed in suspension culture. This model leverages high-throughput sample generation from low-volume patient-derived samples to facilitate the development of personalized medicine treatments. Additionally, co-culture of droplets derived from tumor samples and normal tissues can be utilized to study interactions between tumor and local immune cells.

**Synonyms:** - PD MOS - Patient-Derived MOS - Patient-Derived Micro-organosphere Model