Basecalling

**Semantic type:** Laboratory Procedure

**Definition:** The assignment of the appropriate nucleobase to each peak found in a raw chromatogram (trace) obtained during nucleotide sequencing; use of the one letter abbreviation for each nucleobase generates a data string that represents the actual nucleotide sequence.

**Synonyms:** - Base Call - Base Calling - Basecall - Basecall - Nucleobase Call - Nucleobase Calling