CRISPR Gene Editing

**Semantic type:** Molecular Biology Research Technique

**Definition:** A technique for gene editing that uses a guide DNA and Cas9 enzyme to cut DNA at a precise location. A gene can be disrupted and inactivated following a single cut or a larger deletion can be created with 2 cuts. If a DNA template is included a directed change can be introduced or a new gene can be added.

**Synonyms:** - CRISPR/Cas9 Gene Editing