Semi-Quantitative Reverse Transcriptase Polymerase Chain Reaction

**Semantic type:** Molecular Biology Research Technique

**Definition:** A type of reverse transcriptase-polymerase chain reaction combined with electrophoresis. After real-time PCR reaction, amplified gene products undergo electrophoretic separation and the level of expression of the target gene is determined by measuring the intensity of the gel bands corresponding to the target gene amplicon normalized to a housekeeping, internal control gene.

**Synonyms:** - SEMI-QUANTITATIVE REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION - sqRT-PCR