Shifted Termination Assay

**Semantic type:** Laboratory Procedure

**Definition:** A proprietary multi-base primer extension method for highly sensitive and specific mutation detection. The method uses proprietary reagents to selectively extend mutant- or target-specific primers with multiple nucleotides including fluorescent dye labeled nucleotides. The method enriches mutation signal 20-40 times to increase detection sensitivity. The extended primers are then separated using a capillary sequencer or other type of capillary electrophoresis instrument.

**Synonyms:** - STA