Hi-C Sequencing

**Semantic type:** Molecular Biology Research Technique

**Definition:** A DNA sequencing strategy designed to detect genome-wide chromatin interactions in the nucleus. The method is based on Chromosome Conformation Capture, in which chromatin is crosslinked with formaldehyde, then digested, and re-ligated in such a way that only DNA fragments that are covalently linked together form ligation products. In Hi-C, a biotin-labeled nucleotide is incorporated at the ligation junction, enabling selective purification of chimeric DNA ligation junctions followed by deep sequencing.

**Synonyms:** - Hi-C - Hi-C