Methyl-binding Domain Sequencing

**Semantic type:** Molecular Biology Research Technique

**Definition:** A strategy to obtain DNA sequences from (all) methylated sites. Genomic DNA is randomly fragmented with ultrasonication and then methylated fragments are captured by a protein with high affinity for double-stranded DNA harboring methylated CpGs; non-methylated DNA fragments are washed away. The methylation-enriched fraction is then barcode tagged and sequenced.

**Synonyms:** - MBD-Seq - MBD-Seq