Direct Sequencing of RNA Immunoprecipitates

**Semantic type:** Molecular Biology Research Technique

**Definition:** A method to recover and sequence interaction sites between RNA and specific ribosomal binding proteins. RNA-protein complexes are immunoprecipitated with antibodies targeted to the protein of interest. After RNase digestion, the RNA that was protected by the protein binding is extracted, reverse-transcribed to cDNA, and sequenced.

**Synonyms:** - RIP-Seq - RIP-Seq