Systematic Evolution of Ligands by Exponential Enrichment

**Semantic type:** Molecular Biology Research Technique

**Definition:** A method for isolating single-stranded DNAs or RNAs (aptamers) with high-affinity to a target protein from a large library with random sequences. The target protein is expressed as a fusion with streptavidin-binding peptide and is the mixed with a pooled library of DNA or RNA ligands containing a 14bp randomized region (14N), and a 5 bp barcode that uniquely identifies the individual SELEX sample. Partially nested primers are used in successive SELEX rounds.

**Synonyms:** - SELEX - SELEX