Transposon Sequencing

**Semantic type:** Molecular Biology Research Technique

**Definition:** A method for accurately determining quantitative genetic interactions on a genome-wide scale in microorganisms. It is based on the assembly of a saturated Mariner transposon insertion library. After library selection, changes in frequency of each insertion mutant are determined by sequencing the flanking regions en masse. Insertion site identification can be used to link DNA mutations to phenotypic variants on a genome-wide scale.

**Synonyms:** - Tn-Seq - Tn-Seq