Paired Tumor-Normal DNA Sequencing

**Semantic type:** Molecular Biology Research Technique

**Definition:** A method that uses next generation sequencing technology to determine the DNA sequences of two samples, one derived from a tumor and one from nonmalignant "normal" cells that are both collected from the same individual. Comparison of the sequences in the tumor and normal samples can simultaneously detect disease-specific gene variants and distinguish somatic variants from germline (hereditary) variants.

**Synonyms:** - Paired Tumor-Germline Sequencing - Paired Tumor-Normal DNA Sequence Analysis - Paired Tumor-Normal Sequence Analysis - Parallel Tumor-Normal Sequencing