Multiplexed Error-Robust Fluorescence In Situ Hybridization

**Semantic type:** Laboratory Procedure

**Definition:** A system that leverages the single-molecule fluorescent in situ hybridization (FISH)-based method to perform spatially-resolved single-cell transcriptome profiling for multiple genes of interest. The system leverages combinatorial labeling where each gene in the panel is assigned a unique binary barcode. Then the sample is stained with barcode-specific probes that are subjected sequential rounds of imaging using fluorescently-labeled probes where each round corresponds to one bit in the binary code; thus, multiple rounds of imaging are read to assemble each barcode. Finally, the system can analyze readout errors and either assign the nearest correct barcode or reject background signals.

**Synonyms:** - MERFISH - MERFISH - Multiplexed Error-Robust FISH