Hematoxylin and Eosin Staining Method

**Semantic type:** Laboratory Procedure

**Definition:** Hematoxylin-and-eosin, or H&E, is a routine staining procedure of tissue sections. This staining method uses two separate dyes, one staining the nucleus and the other staining the cytoplasm and connective tissue. Hematoxylin is a dark purplish dye that stains the chromatin (nuclear material) within the nucleus. Eosin is an orangish-pink to red dye that stains the cytoplasmic material including connective tissue and collagen.

**Synonyms:** - H and E - H and E Staining - H and E Staining Method - H&E - H&E Stain - H&E Staining - H&E Staining Method - HEMATOXYLIN AND EOSIN STAIN - Haemotoxylin and Eosin (H&E) - Hematoxylin and Eosin - Hematoxylin and Eosin Staining