Acridine Orange Staining Method

**Semantic type:** Laboratory Procedure

**Definition:** A microscopy staining method that utilizes acridine orange, a fluorescent cationic dye that is selective for nucleic acids, to determine the presence of viable cells or microorganisms in a sample. Acridine orange interacts with RNA and DNA and the lysosomal compartment of cells.

**Synonyms:** - ACRIDINE ORANGE STAIN