Ryan Blue Staining Method

**Semantic type:** Laboratory Procedure

**Definition:** A microscopy staining method that utilizes a modified trichrome stain to determine the presence of microsporidial species. This staining technique substitutes aniline blue for the fast green counterstain of the original trichrome staining method and decreased levels of phosphotungstic acid in the reagent.

**Synonyms:** - RYAN BLUE STAIN