Strand Displacement Amplification

**Semantic type:** Molecular Biology Research Technique

**Definition:** An isothermal, in vitro nucleic acid amplification method that allows primer-directed amplification of a target sequence. It utilizes a primer that can be nicked to create a polymerase start site and a DNA polymerase that will displace a downstream strand as it tracks along the template.

**Synonyms:** - SDA - Strand Displacement Amplification Assay