Multi-Analyte Fluorescent Detection

**Semantic type:** Laboratory Procedure

**Definition:** A method for simultaneous detection of multiple target molecules. It utilizes specific antibodies for the analytes of interest coupled to fluorescent polystyrol beads. After conjugation of the captured analyte to the bead, the beads are differentiated by their size and their spectral signature, allowing quantitation of analyte concentration in the sample.

**Synonyms:** - MAFD - Multi-Analyte Fluorescent Detection Assay