BG8 IHC Staining Method

**Semantic type:** Laboratory Procedure

**Definition:** An immunohistochemical technique utilizing the mouse monoclonal antibody BG8, which is directed against the type 2 fucosylated antigen Lewis Y (LeY). BG8 specifically binds to LeY, a blood group Lewis carbohydrate determinant expressed on certain glycolipids and glycoproteins. In healthy subjects, LeY is expressed in low levels by a few types of epithelial cells; however, it is aberrantly expressed in high levels in many carcinomas. This method is use in the detection of certain tumors, such as carcinomas of the breast, lung, and colon. Additionally, it may distinguish neoplasms derived from mesothelium from other epithelial-derived neoplasms.

**Synonyms:** - Anti-Anti-Lewis Y BG8 Staining Method - Anti-Anti-Lewis-Y BG8 Staining Method - Anti-LeY BG8 Staining Method - Antibody BG8 Staining Method - BG-8 Staining Method - BG8 - BG8 - BG8 Antibody Staining Method - BG8 Staining Method - Lewis Y Antigen Blood Group 8