Immunohistochemistry Staining Method

**Semantic type:** Laboratory Procedure

**Definition:** Immunohistochemical staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

**Synonyms:** - Cell/Tissue, Immunohistochemistry - IHC - IHC - IHC - Immunohistochemistry - Immunohistochemistry - Immunohistochemistry (IHC) - immunohistochemistry