Multiplexed Ion Beam Imaging

**Semantic type:** Laboratory Procedure

**Definition:** A method for multiplexed immunohistochemistry that uses secondary ion mass spectrometry to image antibodies tagged with isotopically pure elemental metal reporters. Multiplexed ion beam imaging (MIBI) is capable of analyzing up to 100 targets simultaneously over a five-log dynamic range. This technique enables comprehensive phenotypic profiling and spatial analysis of the tissue microenvironment.

**Synonyms:** - MIBI - MIBI