Tissue-Based Cyclic Immunofluorescence

**Semantic type:** Laboratory Procedure

**Definition:** A method for immunofluorescence imaging of formalin-fixed, paraffin-embedded (FFPE) specimens mounted on glass slides that generates images using an iterative, cycling process in which conventional fluorescence images capturing up to 60 targets are repeatedly collected from the same sample and then assembled into a high-dimensional representation of the spacial relationships between the multiplex targets.

**Synonyms:** - Tissue Cyclic Immunofluorescence - t-CyCIF - t-CyCIF