Cyclic Immunofluorescence

**Semantic type:** Laboratory Procedure

**Definition:** A method for highly multiplexed immunofluorescent imaging of cells in various formats, such as tissue sections and multi-well tissue culture plates, using fluorescent microscopy. Images are generated using an iterative, cycling process in which images of expressed fluorescently-tagged proteins, fluorescent dyes, or fluorescently-tagged primary or secondary antibodies can be serially collected from the same sample and then those images can be assembled into a high-dimensional representation of the spatial relationships between the targets of interest.

**Synonyms:** - CyCIF - CyCIF - CycIF - CycIF